Helping You Find Full Text Journal Articles

Mar
2017

Download: Single-Cell Transcriptional Analysis.

Annu Rev Anal Chem (Palo Alto Calif) 2017 Mar 16. Epub 2017 Mar 16.
Angela R Wu, Jianbin Wang, Aaron M Streets, Yanyi Huang
Despite being a relatively recent technological development, single-cell transcriptional analysis through high-throughput sequencing has already been used in hundreds of fruitful studies to make exciting new biological discoveries that would otherwise be challenging or even impossible. Consequently, this has fueled a virtuous cycle of even greater interest in the field and compelled development of further improved technical methodologies and approaches. Thanks to the combined efforts of the research community, including the fields of biochemistry and molecular biology, technology and instrumentation, data science, computational biology, and bioinformatics, the single-cell RNA-sequencing field is advancing at a pace that is both astounding and unprecedented.
Full Text Link Source Status
https://dash.harvard.edu/bitstream/handle/1/29626146/4886368
Web SearchFound
http://bioinformatics.oxfordjournals.org/content/31/7/1060.f
Web SearchFound
https://pdfs.semanticscholar.org/165d/d833d2b895abea4b3e597d
Web SearchFound
http://www.annualreviews.org/doi/10.1146/annurev-anchem-0615
Publisher SiteFound


Similar Publications

Mar
2017

A light-addressable potentiometric sensor (LAPS) is a semiconductor-based chemical sensor, in which a measurement site on the sensing surface is defined by illumination. This light addressability can be applied to visualize the spatial distribution of pH or the concentration of a specific chemical species, with potential applications in the fields of chemistry, materials science, biology, and medicine. In this review, the features of this chemical imaging sensor technology are compared with those of other technologies.

View Full Text PDF Listings View primary source full text article PDFs.

Mar
2017

Recent years have witnessed an increased use of droplet-based microfluidic techniques in a wide variety of chemical and biological assays. Nevertheless, obtaining dynamic data from these platforms has remained challenging, as this often requires reading the same droplets (possibly thousands of them) multiple times over a wide range of intervals (from milliseconds to hours). In this review, we introduce the elemental techniques for the formation and manipulation of microfluidic droplets, together with the most recent developments in these areas.

View Full Text PDF Listings View primary source full text article PDFs.

Mar
2017

Capturing the dynamic interplay between proteins and their myriad interaction partners is critically important for advancing our understanding of almost every biochemical process and human disease. The importance of this general area has spawned many measurement methods capable of assaying such protein complexes, and the mass spectrometry-based structural biology methods described in this review form an important part of that analytical arsenal. Here, we survey the basic principles of such measurements, cover recent applications of the technology that have focused on protein-small-molecule complexes, and discuss the bright future awaiting this group of technologies.

View Full Text PDF Listings View primary source full text article PDFs.

Feb
2017

Since its discovery in 1974, surface-enhanced Raman scattering (SERS) has gained momentum as an important tool in analytical chemistry. SERS is used widely for analysis of biological samples, ranging from in vitro cell culture models, ex vivo tissue and blood samples, and direct in vivo application. New insights have been gained into biochemistry, with an emphasis on biomolecule detection, from small molecules such as glucose and amino acids to larger biomolecules such as DNA, proteins, and lipids.

View Full Text PDF Listings View primary source full text article PDFs.
Back to top