Helping You Find Full Text Journal Articles

Search Results:

Author: Lisa J McCawley (16)


Jan
2018

A main goal of mathematical and computational oncology is to develop quantitative tools to determine the most effective therapies for each individual patient. This involves predicting the right drug to be administered at the right time and at the right dose. Such an approach is known as precision medicine.

View Full Text PDF Listings View primary source full text article PDFs.

Dec
2016

Understanding blood-brain barrier responses to inflammatory stimulation (such as lipopolysaccharide mimicking a systemic infection or a cytokine cocktail that could be the result of local or systemic inflammation) is essential to understanding the effect of inflammatory stimulation on the brain. It is through the filter of the blood-brain barrier that the brain responds to outside influences, and the blood-brain barrier is a critical point of failure in neuroinflammation. It is important to note that this interaction is not a static response, but one that evolves over time.

View Full Text PDF Listings View primary source full text article PDFs.

Sep
2015

The blood-brain barrier (BBB) is a critical structure that serves as the gatekeeper between the central nervous system and the rest of the body. It is the responsibility of the BBB to facilitate the entry of required nutrients into the brain and to exclude potentially harmful compounds; however, this complex structure has remained difficult to model faithfully in vitro. Accurate in vitro models are necessary for understanding how the BBB forms and functions, as well as for evaluating drug and toxin penetration across the barrier.

View Full Text PDF Listings View primary source full text article PDFs.

Jun
2015

Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead.

Carcinogenesis 2015 Jun;36 Suppl 1:S254-96
William H Goodson, Leroy Lowe, David O Carpenter, Michael Gilbertson, Abdul Manaf Ali, Adela Lopez de Cerain Salsamendi, Ahmed Lasfar, Amancio Carnero, Amaya Azqueta, Amedeo Amedei, Amelia K Charles, Andrew R Collins, Andrew Ward, Anna C Salzberg, Annamaria Colacci, Ann-Karin Olsen, Arthur Berg, Barry J Barclay, Binhua P Zhou, Carmen Blanco-Aparicio, Carolyn J Baglole, Chenfang Dong, Chiara Mondello, Chia-Wen Hsu, Christian C Naus, Clement Yedjou, Colleen S Curran, Dale W Laird, Daniel C Koch, Danielle J Carlin, Dean W Felsher, Debasish Roy, Dustin G Brown, Edward Ratovitski, Elizabeth P Ryan, Emanuela Corsini, Emilio Rojas, Eun-Yi Moon, Ezio Laconi, Fabio Marongiu, Fahd Al-Mulla, Ferdinando Chiaradonna, Firouz Darroudi, Francis L Martin, Frederik J Van Schooten, Gary S Goldberg, Gerard Wagemaker, Gladys N Nangami, Gloria M Calaf, Graeme Williams, Gregory T Wolf, Gudrun Koppen, Gunnar Brunborg, H Kim Lyerly, Harini Krishnan, Hasiah Ab Hamid, Hemad Yasaei, Hideko Sone, Hiroshi Kondoh, Hosni K Salem, Hsue-Yin Hsu, Hyun Ho Park, Igor Koturbash, Isabelle R Miousse, A Ivana Scovassi, James E Klaunig, Jan Vondráček, Jayadev Raju, Jesse Roman, John Pierce Wise, Jonathan R Whitfield, Jordan Woodrick, Joseph A Christopher, Josiah Ochieng, Juan Fernando Martinez-Leal, Judith Weisz, Julia Kravchenko, Jun Sun, Kalan R Prudhomme, Kannan Badri Narayanan, Karine A Cohen-Solal, Kim Moorwood, Laetitia Gonzalez, Laura Soucek, Le Jian, Leandro S D'Abronzo, Liang-Tzung Lin, Lin Li, Linda Gulliver, Lisa J McCawley, Lorenzo Memeo, Louis Vermeulen, Luc Leyns, Luoping Zhang, Mahara Valverde, Mahin Khatami, Maria Fiammetta Romano, Marion Chapellier, Marc A Williams, Mark Wade, Masoud H Manjili, Matilde E Lleonart, Menghang Xia, Michael J Gonzalez, Michalis V Karamouzis, Micheline Kirsch-Volders, Monica Vaccari, Nancy B Kuemmerle, Neetu Singh, Nichola Cruickshanks, Nicole Kleinstreuer, Nik van Larebeke, Nuzhat Ahmed, Olugbemiga Ogunkua, P K Krishnakumar, Pankaj Vadgama, Paola A Marignani, Paramita M Ghosh, Patricia Ostrosky-Wegman, Patricia A Thompson, Paul Dent, Petr Heneberg, Philippa Darbre, Po Sing Leung, Pratima Nangia-Makker, Qiang Shawn Cheng, R Brooks Robey, Rabeah Al-Temaimi, Rabindra Roy, Rafaela Andrade-Vieira, Ranjeet K Sinha, Rekha Mehta, Renza Vento, Riccardo Di Fiore, Richard Ponce-Cusi, Rita Dornetshuber-Fleiss, Rita Nahta, Robert C Castellino, Roberta Palorini, Roslida Abd Hamid, Sabine A S Langie, Sakina E Eltom, Samira A Brooks, Sandra Ryeom, Sandra S Wise, Sarah N Bay, Shelley A Harris, Silvana Papagerakis, Simona Romano, Sofia Pavanello, Staffan Eriksson, Stefano Forte, Stephanie C Casey, Sudjit Luanpitpong, Tae-Jin Lee, Takemi Otsuki, Tao Chen, Thierry Massfelder, Thomas Sanderson, Tiziana Guarnieri, Tove Hultman, Valérian Dormoy, Valerie Odero-Marah, Venkata Sabbisetti, Veronique Maguer-Satta, W Kimryn Rathmell, Wilhelm Engström, William K Decker, William H Bisson, Yon Rojanasakul, Yunus Luqmani, Zhenbang Chen, Zhiwei Hu
Lifestyle factors are responsible for a considerable portion of cancer incidence worldwide, but credible estimates from the World Health Organization and the International Agency for Research on Cancer (IARC) suggest that the fraction of cancers attributable to toxic environmental exposures is between 7% and 19%. To explore the hypothesis that low-dose exposures to mixtures of chemicals in the environment may be combining to contribute to environmental carcinogenesis, we reviewed 11 hallmark phenotypes of cancer, multiple priority target sites for disruption in each area and prototypical chemical disruptors for all targets, this included dose-response characterizations, evidence of low-dose effects and cross-hallmark effects for all targets and chemicals. In total, 85 examples of chemicals were reviewed for actions on key pathways/mechanisms related to carcinogenesis.

View Full Text PDF Listings View primary source full text article PDFs.

Jun
2015

The purpose of this review is to stimulate new ideas regarding low-dose environmental mixtures and carcinogens and their potential to promote invasion and metastasis. Whereas a number of chapters in this review are devoted to the role of low-dose environmental mixtures and carcinogens in the promotion of invasion and metastasis in specific tumors such as breast and prostate, the overarching theme is the role of low-dose carcinogens in the progression of cancer stem cells. It is becoming clearer that cancer stem cells in a tumor are the ones that assume invasive properties and colonize distant organs.

View Full Text PDF Listings View primary source full text article PDFs.

Dec
1969

The blood-brain barrier (BBB) dynamically controls exchange between the brain and the body, but this interaction cannot be studied directly in the intact human brain or sufficiently represented by animal models. Most existing in vitro BBB models do not include neurons and glia with other BBB elements and do not adequately predict drug efficacy and toxicity. Under the National Institutes of Health Microtissue Initiative, we are developing a three-dimensional, multicompartment, organotypic microphysiological system representative of a neurovascular unit of the brain.

View Full Text PDF Listings View primary source full text article PDFs.

Feb
2014

Polydimethylsiloxane (PDMS) is a commonly used polymer in the fabrication of microfluidic devices due to such features as transparency, gas permeability, and ease of patterning with soft lithography. The surface characteristics of PDMS can also be easily changed with oxygen or low pressure air plasma converting it from a hydrophobic to a hydrophilic state. As part of such a transformation, surface methyl groups are removed and replaced with hydroxyl groups making the exposed surface to resemble silica, a gas impermeable substance.

View Full Text PDF Listings View primary source full text article PDFs.

Nov
2012

We have developed a novel, portable, gravity-fed, microfluidics-based platform suitable for optical interrogation of long-term organotypic cell culture. This system is designed to provide convenient control of cell maintenance, nutrients, and experimental reagent delivery to tissue-like cell densities housed in a transparent, low-volume microenvironment. To demonstrate the ability of our Thick-Tissue Bioreactor (TTB) to provide stable, long-term maintenance of high-density cellular arrays, we observed the morphogenic growth of human mammary epithelial cell lines, MCF-10A and their invasive variants, cultured under three-dimensional (3D) conditions inside our system.

View Full Text PDF Listings View primary source full text article PDFs.

Oct
2011

Morphogenesis is a fundamental process by which new blood vessels are formed during angiogenesis. The ability to control angiogenesis would lead to improvements in tissue engineering constructions; indeed, the study of angiogenesis has numerous clinical applications, for example, in the investigation of metastatic cancer, peripheral and coronary vascular disease, and wound healing. Conventional in vitro organotypic cell culture approaches to these studies are limited primarily by their reliance on microvascular vessel formation through a random process of morphogenesis that lacks the spatial reproducibility and orientation needed for high-throughput drug testing.

View Full Text PDF Listings View primary source full text article PDFs.

Apr
2010

In its simplest description, a tumor is comprised of an expanding population of transformed cells supported by a surrounding microenvironment termed the tumor stroma. The tumor microenvironment has a very complex composition, including multiple types of stromal cells, a dense network of various extracellular matrix (ECM) fibers interpenetrated by the interstitial fluid and gradients of several chemical species that either are dissolved in the fluid or are bound to the ECM structure. In order to study experimentally such complex interactions between multiple players, cancer is dissected and considered at different scales of complexity, such as protein interactions, biochemical pathways, cellular functions or whole organism studies.

View Full Text PDF Listings View primary source full text article PDFs.

Jul
2009

In this paper we consider chemotherapy in a spatial model of tumor growth. The model, which is of reaction-diffusion type, takes into account the complex interactions between the tumor and surrounding stromal cells by including densities of endothelial cells and the extra-cellular matrix. When no treatment is applied the model reproduces the typical dynamics of early tumor growth.

View Full Text PDF Listings View primary source full text article PDFs.

Nov
2008

Matrix metalloproteinase (MMP)-3 is induced by multiple cell types in the skin during processes involved in both normal and pathological tissue remodeling. We previously demonstrated that MMP3-null animals have an increased sensitivity to the development of squamous cell carcinoma, suggesting that overall, MMP3 has a protective role in squamous cell carcinoma. However, not all cellular responses affected by a loss of MMP3 are tumor-protective, and tumor expression of MMP3 is co-incident with an invasive tumor phenotype.

View Full Text PDF Listings View primary source full text article PDFs.

Feb
2005

Matrix metalloproteinase-7 (MMP-7) is primarily expressed in glandular epithelium. Therefore, its mechanism of action may be influenced by its regulated vectorial release to either the apical and/or basolateral compartments, where it would act on its various substrates. To gain a better understanding of where MMP-7 is released in polarized epithelium, we have analyzed its pattern of secretion in polarized MDCK cells expressing stably transfected human MMP-7 (MDCK-MMP-7), and HCA-7 and Caco2 human colon cancer cell lines.

View Full Text PDF Listings View primary source full text article PDFs.

Oct
2004

Elevated expression of matrix metalloproteinase-3 (MMP-3/stromelysin-1) is associated with a variety of tumor types, although its in vivo functional role remains unclear. In human and murine squamous cell carcinoma (SCC), MMP-3 is expressed in the stromal compartment at all of the stages of tumor progression and is expressed by the malignant epithelial cells in late-stage, highly invasive tumors. To elucidate whether MMP-3 plays a causal role during SCC, wild-type and MMP-3 null mice were subjected to chemical carcinogenesis procedures by topical application of either the complete carcinogen 1-methyl-3-nitro-1-nitroso-guanidine or two-stage initiation and promotion with 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate.

View Full Text PDF Listings View primary source full text article PDFs.

Aug
2004

Fetuin, a major serum glycoprotein secreted by the liver, has been shown to play a role in bone development, calcium homeostasis and insulin sensitivity. In an earlier study, we demonstrated that bovine fetuin can bind to the plasma membrane of squamous and spindle-cell carcinoma cells. To test our hypothesis that fetuin plays a causal role in skin tumorigenesis, fetuin-A null and wild-type mice were challenged using a two-stage chemically-induced carcinogenesis protocol with DMBA (7,12-dimethylbenzo(a)anthracene) as the initiator, followed by twice weekly treatments with the tumor promoter TPA (12-O-tetradecanoylphorbol-13-acetate).

View Full Text PDF Listings View primary source full text article PDFs.

Nov
2002

Alpha2-HS glycoprotein (fetuin) is a major plasma glycoprotein predominantly synthesized by the liver. We have previously demonstrated that human fetuin produced by hepatocellular carcinoma cells can activate the matrix metalloproteinase MMP-9 (gelatinase-B). Stromelysin-1 (MMP-3) is over-expressed in murine skin tumors and is associated with a metastatic cell phenotype.

View Full Text PDF Listings View primary source full text article PDFs.

Back to top